1. Growth inhibition data
CSV with two columns: inhibitor_concentration, cell_growth. Header row optional.
Drop a CSV file, or click to browse
.csv · two numeric columns
2. Units
Used to label the axes and the calculated values.
3. Reference controls
Average values for growth and negative control wells. Auto-filled from data, editable.
Use these checkboxes if the data doesn't span enough of the plateau for the model to estimate that asymptote reliably. Note that this will change the analysis so you aren't determining the (relative) IC50. Clicking both checkboxes will calculate the "absolute IC50". Refer to this article for a solid discussion on the difference between relative and absolute IC50.
4. Run
Fits the 4PL and modified Gompertz models and calculates IC50 and MIC.
Dose–response curve
No data yet — load a CSV or the sample dataset, then run the analysis.