|
|
Publications
Dam TK, Brewer CF. Probing lectin-mucin interactions by isothermal
titration microcalorimetry. Methods Mol Biol. 2015;1207:75-90. doi:
10.1007/978-1-4939-1396-1_5.
Talaga ML, Fan N, Fueri AL, Brown RK, Chabre YM, Bandyopadhyay P, Roy
R, Dam TK. Significant other half of a glycoconjugate: contributions of
scaffolds to lectin-glycoconjugate interactions. Biochemistry. 2014 Jul
15;53(27):4445-54. doi: 10.1021/bi5001307. Epub 2014 Jul 3.
Haugstad KE, Gerken TA, Stokke BT, Dam TK, Brewer CF, Sletmoen M.
Enhanced self-association of mucins possessing the T and Tn
carbohydrate cancer antigens at the single-molecule level.
Biomacromolecules. 2012 May 14;13(5):1400-9. doi: 10.1021/bm300135h.
Epub 2012 Apr 3.
Dam TK, Cavada BS, Nagano CS, Rocha BA, Benevides RG, Nascimento KS, de
Sousa LA, Oscarson S, Brewer CF. Fine specificities of two lectins from
Cymbosema roseum seeds: a lectin specific for high-mannose
oligosaccharides and a lectin specific for blood group H type II
trisaccharide. Glycobiology. 2011 Jul;21(7):925-33. doi:
10.1093/glycob/cwr025. Epub 2011 Mar 15.
Dam, T. K. and Brewer, C. F., Maintenance of cell surface glycan
density by lectin-glycan interactions: a homeostatic and innate immune
regulatory mechanism. Glycobiology 20, 1061-1064 (2010).
Dam, T. K. and Brewer, C. F., Multivalent lectin-carbohydrate
interactions energetics and mechanisms of binding. Adv. Carbohydrate
Chem. Biochem. 63, 139-164 (2010).
Dam, T. K. and Brewer, C. F., Lectins as pattern recognition molecules:
the effects of epitope density in innate immunity. Glycobiology 20,
270-279 (2010).
André, S., Denis Gigučre, D., Dam, T. K., Fred Brewer, C. F., Gabius,
H-J. and Roy, R. Synthesis and screening of a small glycomimetic
library for inhibitory activity on medically relevant
galactoside-specific lectins in assays of increasing biorelevance. New
J. Chem., 2010 DOI: 10.1039/c0nj00277a (2010).
Dam, T. K., Gerken, T. A. and Brewer, C. F. Thermodynamics of
multivalent carbohydrate-lectin cross-linking interactions: importance
of entropy in the bind and jump mechanism. Biochemistry, 48,
3822-3827 (2009).
Sletmoen, M., Dam, T. K., Gerken, T. A., Stokke, B. T. and Brewer, C.
F. Single-molecule pair studies of the interactions of the a-GalNAc
(Tn-antigen) form of porcine submaxillary mucin with soybean
agglutinin. Biopolymers, 91, 719-728 (2009).
Szabo, P., Dam, T. K., Smetana, K., Dvoránková, B., Kübler, D., Brewer,
C. F. and Gabius, H.-J., Phosphorylated human lectin galectin-3:
Analysis of ligand binding by histochemical monitoring of
normal/malignant squamous epithelia and by isothermal titration
calorimetry. Anat. Histol. Embryol., 38, 68-75 (2009).
Dam, T. K. and Brewer, C. F., Thermodynamic approaches to the study of
affinity of clustered carbohydrate epitopes in galectin-glycoconjugate
interactions in Animal Lectins: A Functional View, (Vasta and Ahmed
ed.), Taylor and Francis, pp. 31-46 (2009).
Dam, T. K., Torres, M., Brewer, C. F. and Casadevall, A., Isothermal
titration calorimetry reveals differential binding thermodynamics of
variable region-identical antibodies differing in constant region for a
univalent ligand. J. Biol. Chem. 283, 31366-31370 (2008).
Dam, T. K., and Brewer, C. F., Effects of clustered epitopes in
multivalent ligand-receptor interactions. Biochemistry, 47, 8470-8476
(2008).
Kübler, D., Hung, C. W., Dam, T. K., Kopitz, J., André, S., Kaltner,
H., Lohr, M., Manning, J. C., He, L., Wang, H., Middelberg, A., Brewer,
C. F., Reed, J., Lehmann, W. D. and Gabius, H. J. Phosphorylated human
galectin-3: facile large-scale preparation of active lectin and
detection of structural changes by CD spectroscopy. Biochim. Biophys.
Acta. 1780, 716-722 (2008).
Dam, T. K., Gerken, T. A., Cavada, B. S., Nascimento, K. S., Moura, T.
R., and Brewer, C. F., Binding studies of a-GalNAc specific lectins to
the a-GalNAc (Tn-antigen) form of porcine submaxillary mucin and its
smaller fragments. J. Biol. Chem. 282, 28256-28263 (2007).
Dam, T. K. and Brewer, C. F. Fundamentals of lectin-carbohydrate
interactions, in Comprehensive Glycoscience, (Kamerling, J. P., ed.),
Elsevier Ltd., Vol. 3, pp. 397-452 (2007).
Dam, T. K. and Brewer, C. F. Applications of isothermal titration calorimetry to lectin-
carbohydrate interactions, in Lectins: Analytical Technologies, (Nilsson, C., ed.),
Elsevier B.V., pp. 75-101 (2007).
Dam, T. K., Gabius, H.-J., André, S., Kaltner, H., Lensch, M., and
Brewer, C. F. Galectins bind to the multivalent glycoprotein
asialofetuin with enhanced affinities and a gradient of decreasing
binding constants. Biochemistry, 44, 12564-12571 (2005).
Dam, T. K., Oscarson, S., Roy, R., Das, S. K., Page, D., Macaluso, F.
and Brewer, C. F. Thermodynamic, kinetic and electron microscopy
studies of concanavalin A and Dioclea grandiflora lectin cross-linked
with synthetic divalent carbohydrates. J. Biol. Chem. 280, 8640-8646
(2005).
Dam, T. K. and Brewer, C. F. Multivalent protein-carbohydrate
interactions: isothermal titration microcalorimetry studies.
Methods in Enzymology (Energetics of Biological Macromolecules, Part D;
J. M. Holt, M. L. Johnson & G.K. Ackers, ed.), 379, 107-128 (2004).
Zheng, R., Dam, T. K., Brewer, C.F. and Blanchard, J. S. Active site
residues in Mycobacterium tuberculosis pantothenate synthetase required
in the formation and stabilization of the adenylate intermediate.
Biochemistry 43, 7171-7178 (2004).
Dam, T. K. and Brewer C. F. Carbohydrate-lectin cross-linking
interactions: structural, thermodynamic, and biological studies.
Methods in Enzymology (Recognition of Carbohydrates in Biological
Systems; Y.C. Lee & R. T. Lee, ed.), 362, 455-486 (2003).
Patil AR, Misquith S, Dam T. K., Sharma V, Kapoor M and Surolia
A. Exploring enzyme amplification to characterize specificities of
protein-carbohydrate recognition. Methods in Enzymology (Recognition of
Carbohydrates in Biological Systems; Y.C. Lee & R. T. Lee, ed.);
362, 567-583 (2003).
Dam, T. K., Roy, R., Page, D. and Brewer, C. F. Negative cooperativity
associated with binding of multivalent carbohydrates to lectins.
Thermodynamic analysis of the “Multivalency Effect”. Biochemistry 41,
1351-1358 (2002).
Dam, T. K., Roy, R., Page, D. and Brewer, C. F. Thermodynamic binding
parameters of individual epitopes of multivalent carbohydrates to
concanavalin A as determined by “reverse” isothermal titration
microcalorimetry. Biochemistry 41, 1359-1363 (2002).
Hegde, S. S., Dam, T. K., Brewer C. F. and Blanchard, J. S.
(2002), Thermodynamics of aminoglycoside and acyl-coenzyme A binding to
the Salmonella enterica AAC(6’)-Iy aminoglycoside N-acetyltransferase.
Biochemistry 41, 7519-7527 (2002).
Dam, T. K. and Brewer, C. F. Thermodynamic studies of
lectin-carbohydrate interactions by isothermal titration calorimetry.
Chem. Rev. 102, 387-429 (2002).
Bachhawat, K., Kapoor, M., Dam, T.K. and Surolia, A. The
reversible two states unfolding of a monocot mannose-binding lectin
from garlic bulbs reveals the dominant role of the dimeric interface in
its stabilization. Biochemistry 40, 7291-7300 (2001).
Cunto-Amesty, G., Dam, T.K., Lou, P., Monzavi-Karbassi, B., Brewer, C.
F. Van Cott, T. C. and Kieber-Emmons, T., Directing the immune response
to carbohydrate antigens. J. Biol. Chem. 276, 30490-30498 (2001).
Dam, T. K., Roy, R., Das, S. K., Oscarson, S. and Brewer, C. F.
Binding of multivalent carbohydrates to concanavalin A and Dioclea
grandiflora lectin.
Thermodynamic analysis of the “Multivalency Effect”. J. Biol. Chem. 275, 14223-14230 (2000).
Dam, T. K., Cavada, B. S., Grangeiro, T. B., Santos, C. F.,
Ceccatto V. M., de Sousa, F. A. M., Oscarson, S. and Brewer, C. F.
Thermodynamic binding studies of lectins from the Diocleinae subtribe
to deoxy analogs of the core trimannoside of asparagine-linked
oligosaccharides. J. Biol. Chem. 275, 16119-16126 (2000).
Roy, R., Das, S. K., Santoyo-Gonzalez, F., Hernandez-Mateo, F.,
Dam, T. K., and Brewer, C. F. Synthesis of sugar-rods with
phytohemagglutinin cross-linking properties by using the
palladium-catalyzed sonogashira reaction. Chem. Eur. J. 6, 1757-1762
(2000).
Lee, H. C., Goroncy, A. K., Peisach, J., Cavada, B. S., Grangeiro, T.
B., Ramos, M. V., Sampaio A. H., Dam, T. K., and Brewer, C. F.
Demonstration of a conserved histidine and two water ligands at the
Mn2+ site in Diocleinae lectins by pulsed EPR spectroscopy.
Biochemistry 39, 2340-2346 (2000).
Chandra, N. R., Ramachandraiah, G., Bachhawat, K., Dam, T. K., Surolia,
A. and Vijayan, M. Crystal structure of a dimeric mannose-specific
agglutinin from garlic: quaternary association and carbohydrate
specificity. J. Mol. Biol. 285, 1157-1168 (1999).
Dam, T. K., Oscarson, S. and Brewer, C. F. Thermodynamics of
binding of the core trimannoside of asparagine-linked carbohydrates and
deoxy analogs to Dioclea grandiflora lectin. J. Biol. Chem. 273,
32812-32817 (1998).
Dam, T. K., Oscarson, S., Sacchettini, J. C. and Brewer, C. F.
Differential solvation of “core” trimannoside complexes of the Dioclea
grandiflora lectin and concanavalin A detected by primary solvent
isotope effects in isothermal titration microcalorimetry. J.
Biol. Chem. 273, 32826-32832 (1998).
Dam, T. K., Cavada, B. S., Grangeiro, T. B., Santos, C. F., de
Sousa, F. A. M., Oscarson, S. and Brewer, C. F. Diocleinae lectins are
a group of proteins with conserved binding sites for the core
trimannoside of asparagine-linked oligosaccharide and differential
specificities for complex carbohydrates. J. Biol. Chem. 273,
12082-12088 (1998).
Dam, T. K., Bachhawat, K., Rani, P. G. and Surolia, A. Garlic
(Allium sativum) lectins bind to high mannose oligosaccharide chains.
J. Biol. Chem. 273, 5528-5535 (1998).
Gupta, D., Dam, T. K., Oscarson, S. and Brewer, C. F. Thermodynamics of
lectin- carbohydrate interactions. Binding of the core trimannoside of
asparagine-linked carbohydrates and deoxy analogs to concanavalin A. J.
Biol. Chem. 272, 6388-6392 (1997).
Chandra, N. R., Dam, T.K., Surolia, A. and Vijayan, M.
Crystallization and preliminary crystallographic studies on the mannose
specific lectin from garlic. Acta Cryst. D53, 787-788 (1997).
Dam, T. K.*, Bandyopadhyay, P., Sarkar, M., Ghosal, J., Bhattacharya,
A. and Choudhury, A. Purification and partial characterization of a
heparin-binding lectin from the marine clam Anadara granosa. Biochem
Biophys. Res. Commun. 203, 36-45 (1994).
Dam, T. K., Sarkar, M., Ghosal, J. and Choudhury, A. Purification
and partial characterization of a N-glycolylneuraminic acid specific
lectin from the clam Anadara granosa (L). Biochem. Biophys. Res.
Commun. 196, 422-429 (1993).
Dam, T. K., Sarkar, M., Ghosal, J. and Choudhury, A. A Novel galactosyl
binding lectin from the plasma of the blood clam, Anadara granosa (L)
and a study of its combining site. Mol. Cell. Biochem. 117, 1-9 (1992).
Book Review: Brewer, C. F. and Dam, T. K. Reviewed the book
entitled “Essentials of Glycobiology” Edited by Varki et al., (Cold
Spring Harbor Laboratory Press, Cold Spring Harbor, New York.). The
review was published in Carbohydrate Research. 325, 233-234 (2000).
|
